The Conus snail (Gastropoda: Conidae) distributes mostly in warm tropical seas and are considered asprecious pharmaceuticals. The aim of this study is to determind the association between the feeding mode and phylogenetic relationships of cone snail, Sequences of gen CO1 mtDNA of present cone snail (25 species) were combined with 19 sequences from Genbank to construct a phylogenetic tree using three analysis methods of maximum parsimony, maximum likelihood and Baysian inference. Phylogenetic analysis based on CO1 mtDNA exhibited the clear association of feeding mode and phylogenetic relationships. Conus species with similar feeding mode were clustered in the same groups: group 1,2,8 amd 10 include the worm eating species; group 3,6 contain molusc eating species fish eating species and those reported having both fish and worm or fish and molusc depending on their lyfe cycle were clustered