Identify and characterize the SNP markers for traceability of scallop spiny lobster Panulirus homarus (Decapoda, Palinuridae) in Vietnam

N N T Nhon1, T Q Sang2, N M Chau1 and D T Binh2
Research Institute of Aquaculture, Nha Trang, Vietnam
2 Institute for Biotechnology and Environment, Nha Trang, Vietnam

Lobster is an aquatic species of ecological and economic importance. In Vietnam, ornate spiny lobster (Panulirus ornatus) and scallop spiny lobster (P. hormatus) are among the main species of aquaculture in Vietnam. Demand for lobster products domestically and internationally is increasing, as in the US, Japan and China markets. Today, consumers are more aware of product brands, food safety and hygiene, so product traceability is essential to protect consumers and businesses. The DNA markers are widely used for commercially traded seafood traceability. Among that, Single Nucleotide Polymorphisms (SNPs) have been shown to be robust markers, and offer high reliability. EzRAD sequencing is applied to detect potential SNPs that are characteristic for P. homarus populations in Vietnam and Sri Lanka. Through 211 valid SNPs, we detected and 12 SNPS occurring in Vietnam population, and designed 8 primer pairs to amplified 80-130 bp SNPs products. PCR optimization allows us to select 2 SNPs serving the traceability of Vietnamese P. homarus. Further need to more population sampling, and whole genome sequencing in order to develop SNPs panel to seeking the origin of lobster seafood products.